St John's Wort Research Today is a free monthly online journal that collates and summarizes the latest research about St John's Wort, including details on depression, anxiety, side-effects, dosage, benefits. | ||||||||
|
The Lipophilic Extract of Hypericum perforatum Exerts Significant Cytotoxic Activity Against T24 and NBT-II Urinary Bladder Tumor Cells.Skalkos D, Stavropoulos NE, Tsimaris I, Gioti E, Stalikas CD, Nseyo UO, Ioachim E, Agnantis NJ Department of Material Sciences & Engineering, University of Ioannina, Ioannina, Greece. Hypericum perforatum L . (St. John's wort) is a medicinal plant used for many pathologies, especially for the treatment of mild to moderate depression. In the present study we have investigated the cytotoxic activity of the locally collected (Epirus region) Hypericum perforatum L . against cultured T24 and NBT-II bladder cancer cell lines. The lipophilic extract of the herb, prepared using petroleum ether, induced apoptosis displaying LC (50) values at concentrations as low as 4 and 5 mug/mL. A fraction of this extract displayed 60 % cell growth inhibition at a concentration of 0.95 mug/mL. Evaluating the importance of various biologically active components of the extract, it was found that hypericins (hypericin, pseudohypericin, etc.) were identified only in the methanolic (lipophobic) extract of the herb, and not in the active lipophilic extract. In addition, hyperforin concentrations in the lipophilic extract and its most active fraction, were 0.94 mug/mL, and 0.17 mug/mL, respectively, while the active cytotoxic concentration of pure hyperforin appeared in the range of 1.8 mug/mL - 5.0 mug/mL. Therefore, pure hyperforin does not seem to contribute significantly to the cytotoxicity activity. Chlorophylls were identified in low, not significantly different, concentrations in all extracts and fractions and were not correlated to the biological activity. Owing to the combination of significant cytotoxic activity, natural abundance and low toxicity, the lipophilic extract of Hypericum perforatum holds the promise of being an interesting, new, antiproliferative agent against bladder cancer that deserves further investigation. Abbreviations. HP: Hypericum perforatum L. AS:aqueous solution MS:methanolic solution ME:methanolic extract NPMF:non polar methanolic fraction PMF:polar methanolic fraction PEE:petroleum ether (lipophilic) extract PEE (1,2,3,4,5,6,7):petroleum ether extract's fractions PBS:phosphate buffered saline DMSO:dimethyl sulfoxide. Published 1 December 2005 in Planta Med, 71(11): 1030-5.
© 2004-2008 St John's Wort Research Today. All Rights Reserved. |
| ||||||
